ChIP-chip for genome-wide analysis of protein binding in mammalian cells.

TitleChIP-chip for genome-wide analysis of protein binding in mammalian cells.
Publication TypeJournal Article
Year of Publication2007
AuthorsKim T H, Barrera LO, Ren B
JournalCurr Protoc Mol Biol
VolumeChapter 21
PaginationUnit 21.13
Date Published2007 Jul
ISSN1934-3647
KeywordsAnimals, Cell Line, Chromatin Immunoprecipitation, DNA, Genomics, Humans, Mammals, Oligonucleotide Array Sequence Analysis, Protein Binding, Proteins, Proteomics
Abstract

ChIP-chip combines chromatin immunoprecipitation (ChIP) with microarrays (chip) to determine protein-DNA interactions occurring in living cells. The high throughput nature of this method makes it an ideal approach for identifying transcription factor targets or chromatin modification sites along the genome. UNIT 21.9 describes a protocol for analysis of protein-DNA interactions in yeast cells. This unit introduces an alternative protocol developed for mammalian cells.

DOI10.1002/0471142727.mb2113s79
PubMed URLhttp://www.ncbi.nlm.nih.gov/pubmed/18265397?dopt=Abstract
Alternate TitleCurr Protoc Mol Biol
PubMed ID18265397