Characterization and modelling of interspecies electron transfer mechanisms and microbial community dynamics of a syntrophic association.

TitleCharacterization and modelling of interspecies electron transfer mechanisms and microbial community dynamics of a syntrophic association.
Publication TypeJournal Article
Year of Publication2013
AuthorsNagarajan H, Embree M, Rotaru A-E, Shrestha PM, Feist AM, Palsson BØ, Lovley DR, Zengler K
JournalNat Commun
Volume4
Pagination2809
Date Published2013 Nov 22
ISSN2041-1723
Abstract

Syntrophic associations are central to microbial communities and thus have a fundamental role in the global carbon cycle. Despite biochemical approaches describing the physiological activity of these communities, there has been a lack of a mechanistic understanding of the relationship between complex nutritional and energetic dependencies and their functioning. Here we apply a multi-omic modelling workflow that combines genomic, transcriptomic and physiological data with genome-scale models to investigate dynamics and electron flow mechanisms in the syntrophic association of Geobacter metallireducens and Geobacter sulfurreducens. Genome-scale modelling of direct interspecies electron transfer reveals insights into the energetics of electron transfer mechanisms. While G. sulfurreducens adapts to rapid syntrophic growth by changes at the genomic and transcriptomic level, G. metallireducens responds only at the transcriptomic level. This multi-omic approach enhances our understanding of adaptive responses and factors that shape the evolution of syntrophic communities.

DOI10.1038/ncomms3809
PubMed URLhttp://www.ncbi.nlm.nih.gov/pubmed/24264237?dopt=Abstract
Alternate JournalNat Commun
PubMed ID24264237