The Clothes Make the mRNA: Past and Present Trends in mRNP Fashion.

TitleThe Clothes Make the mRNA: Past and Present Trends in mRNP Fashion.
Publication TypeJournal Article
Year of Publication2015
AuthorsSingh G, Pratt G, Yeo GW, Moore MJ
JournalAnnu Rev Biochem
Volume84
Pagination325-54
Date Published2015
ISSN1545-4509
KeywordsActive Transport, Cell Nucleus, Animals, Humans, Protein Biosynthesis, Ribonucleoproteins, RNA Splicing, RNA Stability, RNA, Messenger, Transcription, Genetic
Abstract

Throughout their lifetimes, messenger RNAs (mRNAs) associate with proteins to form ribonucleoproteins (mRNPs). Since the discovery of the first mRNP component more than 40 years ago, what is known as the mRNA interactome now comprises >1,000 proteins. These proteins bind mRNAs in myriad ways with varying affinities and stoichiometries, with many assembling onto nascent RNAs in a highly ordered process during transcription and precursor mRNA (pre-mRNA) processing. The nonrandom distribution of major mRNP proteins observed in transcriptome-wide studies leads us to propose that mRNPs are organized into three major domains loosely corresponding to 5' untranslated regions (UTRs), open reading frames, and 3' UTRs. Moving from the nucleus to the cytoplasm, mRNPs undergo extensive remodeling as they are first acted upon by the nuclear pore complex and then by the ribosome. When not being actively translated, cytoplasmic mRNPs can assemble into large multi-mRNP assemblies or be permanently disassembled and degraded. In this review, we aim to give the reader a thorough understanding of past and current eukaryotic mRNP research.

DOI10.1146/annurev-biochem-080111-092106
PubMed URLhttp://www.ncbi.nlm.nih.gov/pubmed/25784054?dopt=Abstract
Alternate JournalAnnu. Rev. Biochem.
PubMed ID25784054
PubMed Central IDPMC4804868
Grant ListGM53007 / GM / NIGMS NIH HHS / United States
HG004659 / HG / NHGRI NIH HHS / United States
NS075449 / NS / NINDS NIH HHS / United States
R01 GM053007 / GM / NIGMS NIH HHS / United States
R01 HG004659 / HG / NHGRI NIH HHS / United States
R01 NS075449 / NS / NINDS NIH HHS / United States
U54HG007005 / HG / NHGRI NIH HHS / United States
/ / Howard Hughes Medical Institute / United States
Track(s): 
Bioinformatics and Systems Biology